Extraction ProcedureThe Randall Hot Extraction ProcedureThis section presents technological advances made inextraction of algae oil. The algae can be extract using differentsolvents according to Randall Hot Extraction Procedure.The Randall Hot Extraction Procedure consists of three steps,Decocting, rinsing and evaporation.1. DecoctingDuring the first step, the extraction thimble containing thesample is immersed in the boiling solvent in the beaker - justlike a tea bag in a cup of hot water. Much of the algae that areto be extracted will already dissolve during this step, anddisperse in the solvent.The upper part of the apparatus just works as a refluxcondenser; the reflux will drip into the thimble, thus helping todissolve the algae.2. RinsingIn the second step, the extraction thimble is lifted out of thebeaker. It still contains some adhering extract; and it might stillcontain some algae that have not yet been dissolved. Now thereflux from the condenser will rinse the adhering extract andalso, given time, dissolve the parts of the algae that are yetundissolved.3. EvaporationIn order to evaporate the solvent, the reflux stopcock closesbelow the condenser. Now the condensate will gather in thelower part of the condenser; we can use it again for the nextextraction. Due to the short distances in the apparatus, it ispossible to evaporate the sample almost to dryness.References:•http://www.behr-labor.com/indexeng.html•Rahmanpour O., Ajami H., Shariati A., Helaleh A.H., Comparison of different solvents used in nanochloropsis algae oil extraction., Journal of Biodiversity and Environmental Sciences., Vol. 5, No. 5, p. 234-240, 2014